Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-9 (of 9 Records) |
Query Trace: Changayil S[original query] |
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Correction for Weigand et al., Complete Genome Sequences of Two Bordetella hinzii Strains Isolated from Humans.
Weigand MR , Changayil S , Kulasekarapandian Y , Batra D , Loparev V , Juieng P , Rowe L , Sheth M , Davis JK , Tondella ML . Genome Announc 2016 4 (1) Volume 3, no. 4, e00965-15, 2015. Page 1: The byline and affiliation line should read as given above. |
Finished Annotated Genome Sequence of Burkholderia pseudomallei Strain Bp1651, a Multidrug-Resistant Clinical Isolate.
Bugrysheva JV , Sue D , Hakovirta J , Loparev VN , Knipe K , Sammons SA , Ranganathan-Ganakammal S , Changayil S , Srinivasamoorthy G , Weil MR , Tatusov RL , Gee JE , Elrod MG , Hoffmaster AR , Weigel LM . Genome Announc 2015 3 (6) Burkholderia pseudomallei strain Bp1651, a human isolate, is resistant to all clinically relevant antibiotics. We report here on the finished genome sequence assembly and annotation of the two chromosomes of this strain. This genome sequence may assist in understanding the mechanisms of antimicrobial resistance for this pathogenic species. |
Complete Genome Sequences of Two Bordetella hinzii Strains Isolated from Humans.
Weigand MR , Changayil S , Kulasekarapandian Y , Tondella ML . Genome Announc 2015 3 (4) Bordetella hinzii is primarily recovered from poultry but can also colonize mammalian hosts and immunocompromised humans. Here, we report the first complete genome sequences of B. hinzii in two isolates recovered from humans. The availability of these sequences will hopefully aid in identifying host-specific determinants variably present within this species. |
The mitochondrial genome of the lone star tick (Amblyomma americanum).
Williams-Newkirk AJ , Burroughs M , Changayil SS , Dasch GA . Ticks Tick Borne Dis 2015 6 (6) 793-801 Amblyomma americanum is an abundant tick in the southeastern, midwestern, and northeastern United States. It is a vector of multiple diseases, but limited genomic resources are available for it. We sequenced the complete mitochondrial genome of a single female A. americanum collected in Georgia using the Illumina platform. The consensus sequence was 14,709bp long, and the mean coverage across the assembly was >12,000x. All expected tick genomic features were present, including two "Tick-Box" motifs, and in the expected order for the Metastriata. Heteroplasmy rates were low compared to the most closely related tick for which data are available, Amblyomma cajennense. The phylogeny derived from the concatenated protein coding and rRNA genes from the 33 available tick mitochondrial genomes was consistent with those previously proposed for the Acari. This is the first complete mitochondrial sequence for A. americanum, which provides a useful reference for future studies of A. americanum population genetics and tick phylogeny. |
Chlamydia psittaci Comparative Genomics Reveals Intraspecies Variations in the Putative Outer Membrane and Type III Secretion System Genes.
Wolff BJ , Morrison SS , Pesti D , Ganakammal SR , Srinivasamoorthy G , Changayil S , Weil MR , MacCannell D , Rowe L , Frace M , Ritchie BW , Dean D , Winchell J . Microbiology (Reading) 2015 161 (7) 1378-91 Chlamydia psittaci is an obligate intracellular bacterium that can cause significant disease among a broad range of hosts. In humans, this organism may cause psittacosis, a respiratory disease that can spread to involve multiple organs, and in rare untreated cases may be fatal. There are ten known genotypes based on sequencing the major outer membrane protein gene, ompA, of C. psittaci. Each genotype has overlapping host preferences and virulence characteristics. Recent studies have compared C. psittaci among other members of the Chlamydiaceae family and showed that this species frequently switches hosts and has undergone multiple genomic rearrangements. In this study, we sequenced five genomes of C. psittaci strains representing four genotypes, A, B, D and E. Due to the known association of the type III secretion system (T3SS) and polymorphic outer membrane proteins (pmps) with host tropism and virulence potential, we performed a comparative analysis of these elements among these five strains along with a representative genome from each of the remaining six genotypes previously sequenced. We found significant genetic variation in the pmps and T3SS genes that may partially explain differences noted in C. psittaci host infection and disease. |
Genome Sequences of 228 Shiga Toxin-Producing Escherichia coli Isolates and 12 Isolates Representing Other Diarrheagenic E. coli Pathotypes.
Trees E , Strockbine N , Changayil S , Ranganathan S , Zhao K , Weil R , MacCannell D , Sabol A , Schmidtke A , Martin H , Stripling D , Ribot EM , Gerner-Smidt P . Genome Announc 2014 2 (4) Shiga toxin-producing Escherichia coli (STEC) are a common cause for food-borne diarrheal illness outbreaks and sporadic cases. Here, we report the availability of the draft genome sequences of 228 STEC strains representing 32 serotypes with known pulsed-field gel electrophoresis (PFGE) types and epidemiological relationships, as well as 12 strains representing other diarrheagenic E. coli pathotypes. |
Whole-genome analysis of Exserohilum rostratum from an outbreak of fungal meningitis and other infections.
Litvintseva AP , Hurst S , Gade L , Frace MA , Hilsabeck R , Schupp JM , Gillece JD , Roe C , Smith D , Keim P , Lockhart SR , Changayil S , Weil MR , MacCannell DR , Brandt ME , Engelthaler DM . J Clin Microbiol 2014 52 (9) 3216-22 Exserohilum rostratum was the cause of most cases of fungal meningitis and other infections associated with the injection of contaminated methylprednisolone acetate produced by the New England Compounding Center (NECC). Until this outbreak, very few human cases of Exserohilum had been reported and very little was known about this dematiaceous fungus, which usually infects plants. Here we report using whole genome sequencing (WGS) for detection of single nucleotide polymorphisms (SNP) and phylogenetic analysis to investigate molecular origin of the outbreak using 22 isolates of E. rostratum isolated from 19 case-patients with meningitis or epidural/spinal abscesses, six isolates isolated from contaminated NECC vials, and seven isolates that are unrelated to the outbreak. Our analysis indicates that all 28 isolates associated with the outbreak had nearly identical genomes of 33.8 Mb. A total of eight SNPs were detected among the outbreak genomes, with no more than two SNPs separating any two of the 28 genomes. The outbreak genomes were separated from the next most closely related control strain by approximately 136,000 SNPs. We also observed significant genomic variability among strains unrelated to the outbreak, which may suggest a possibility of cryptic speciation in E. rostratum. |
Microbial biofilms on needleless connectors for central venous catheters: a comparison of standard and silver-coated devices collected from patients in an acute care hospital
Perez E , Williams M , Jacob JT , Reyes MD , Chernetsky Tejedor S , Steinberg JP , Rowe L , Ganakammal SR , Changayil S , Weil MR , Donlan RM . J Clin Microbiol 2013 52 (3) 823-31 Microorganisms may colonize needleless connectors (NCs) on intravascular catheters, forming biofilms and predisposing patients to catheter- associated infection (CAI). Standard and silver-coated NCs were collected from catheterized intensive care unit patients to characterize biofilm formation using culture-dependent and culture-independent methods and to investigate association between NC usage and biofilm characteristics. Viable microorganisms were detected by plate count (PC) from 46% of standard and 59% of silver-coated NCs (p=0.11). There were no significant associations (p>0.05, chi-squared test) between catheter type, side of catheter placement, number of catheter lumens, site of catheter placement, or NC duration, and positive NC. There was an association (p=0.04, chi-squared test) between infusion type and positive standard NCs. Viable microorganisms exhibiting intracellular esterase activity were detected on >90% of both NC types (p=0.751), suggesting that a large percentage of organisms were not culturable using the conditions provided in this study. Amplification of the 16S ribosomal RNA gene from selected NCs provided a substantially larger number of operational taxonomic units per NC than PC (26-43 vs 1-4), suggesting that culture-dependent methods may substantially underestimate microbial diversity on NCs. NC bacterial communities were clustered by patient and venous access type and may reflect the composition of the patient's local microbiome but may also contain organisms from the healthcare environment. NCs provide a portal of entry for a wide diversity of opportunistic pathogens to colonize the catheter lumen, forming a biofilm and increasing the potential for CAI, highlighting the importance of catheter maintenance practices to reduce microbial contamination. |
Draft genome sequences of Bordetella holmesii strains from blood (F627) and nasopharynx (H558).
Tatti KM , Loparev VN , Ranganathanganakammal S , Changayil S , Frace M , Weil MR , Sammons S , Maccannell D , Mayer LW , Tondella ML . Genome Announc 2013 1 (2) e0005613 Bordetella holmesii, a human pathogen, can confound the diagnosis of respiratory illness caused by Bordetella pertussis. We present the draft genome sequences of two B. holmesii isolates, one from blood, F627, and one from the nasopharynx, H558. Interestingly, important virulence genes that are present in B. pertussis are not found in B. holmesii. |
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